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Vrije Universiteit Brussel

6 ARTICLES PUBLISHED IN JoVE

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Biology

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
Lise Schotte 1, Bart Rombaut 1, Bert Thys 1
1Department of Pharmaceutical Biotechnology and Molecular Biology, Centre for Pharmaceutical Research, Vrije Universiteit Brussel

In this article, a simple, quantitative, liquid phase affinity capture assay is presented. It is a reliable technique based on the interaction between magnetic beads and tagged proteins (e.g. nanobodies) on one hand and the affinity between the tagged protein and a second, labeled protein (e.g. poliovirus) on the other.

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Medicine

Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation
Sofie De Groef 1, Gunter Leuckx 1, Naomi Van Gassen 1, Willem Staels 1, Ying Cai 1, Yixing Yuchi 1, Violette Coppens 1, Nico De Leu 1, Yves Heremans 1, Luc Baeyens 1, Mark Van de Casteele 1, Harry Heimberg 1
1Diabetes Research Center, Vrije Universiteit Brussel

This protocol describes an injury model involving the surgical ligation of the pancreatic duct in the adult mouse pancreas, resulting in severe injury that establishes an environment that allows beta cell neogenesis and proliferation. This model can be used as a tool to study mechanisms involved in beta cell formation.

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Medicine

Dynamic Lung Tumor Tracking for Stereotactic Ablative Body Radiation Therapy
Charles A. Kunos 1, Jeffrey M. Fabien 1, John P. Shanahan 1, Christine Collen 2, Thierry Gevaert 2, Kenneth Poels 2, Robbe Van den Begin 2, Benedikt Engels 2, Mark De Ridder 2
1Department of Radiation Oncology, Summa Cancer Institute, 2Department of Radiation Oncology, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel

Stereotactic ablative body radiation therapy (SBRT) involves the precise delivery of high-dose radiation to cancer tumor targets. A novel SBRT platform offers a first-of-its-kind gimbaled radiation accelerator mounted within a pivoting O-ring gantry capable of dynamic image-guided tumor tracking. Here, we describe dynamic tumor tracking for lung targets.

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Engineering

Emission Spectroscopic Boundary Layer Investigation during Ablative Material Testing in Plasmatron
Bernd Helber 1,2, Olivier Chazot 1, Annick Hubin 2, Thierry E. Magin 1
1Aeronautics and Aerospace Department, von Karman Institute for Fluid Dynamics, 2Research Group Electrochemical and Surface Engineering, Vrije Universiteit Brussel

Development of new ablative materials and their numerical modeling requires extensive experimental investigation. This protocol describes procedures for material response characterization in plasma flows with the core techniques being non-intrusive methods to track the material recession along with the chemistry in the reactive boundary layer by emission spectroscopy.

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Medicine

Non-invasive Assessments of Subjective and Objective Recovery Characteristics Following an Exhaustive Jump Protocol
Erich Hohenauer 1,2,3, Peter Clarys 3, Jean-Pierre Baeyens 2,3,4, Ron Clijsen 1,2,3
1Department of Business Economics, Health and Social Care, University of Applied Sciences and Arts of Southern Switzerland, 2University College Physiotherapy "Thim van der Laan", 3Department of Movement and Sport Sciences, Vrije Universiteit Brussel, 4Faculty of Medicine and Health Sciences, University of Antwerp

This protocol describes the procedure for non-invasive recovery assessments during a 72 h recovery period. This protocol induces muscular exhaustion of the frontal thighs using countermovement jumps and uses either local cold-cuff or thermoneutral-cuff application as a recovery modality.

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Ovarian Tissue Oocyte-<em>In Vitro</em> Maturation for Fertility Preservation

Ovarian Tissue Oocyte-In Vitro Maturation for Fertility Preservation
Ingrid Segers 1, Ileana Mateizel 1, Koen Wouters 1, Ellen Van Moer 1, Ellen Anckaert 2, Neelke De Munck 1, Michel De Vos 1,2
1Brussels IVF, University Hospital Brussels, Vrije Universiteit Brussel, 2Follicle Biology Laboratory (FOBI), Vrije Universiteit Brussel

Presented here is ovarian tissue oocyte-in vitro maturation (OTO-IVM), an accessible technique within a medical assisted reproduction (MAR) laboratory offering realistic additional fertility preservation options to patients who need ovarian tissue cryopreservation.

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