Yamaguchi University

2 ARTICLES PUBLISHED IN JoVE

Behavior

Four Temporary Waterslide Designs Adapted to Different Slope Conditions to Encourage Child Socialization in Playgrounds

Zhudi Hua¹, Ting Tao¹, Risa Akita¹, Tomofusa Akita¹, Yoshiaki Hayakawa¹, Masanori Hariyama², Hayato Sakurai³, Ricki Colman⁴, Mamiko Koshiba^1,2,3

¹Graduate School of Sciences and Technology for Innovation, Yamaguchi University, ²Graduate School of Information Sciences, Tohoku University, ³Pediatrics, Saitama Medical University Hospital, ⁴Department of Cell and Regenerative Biology, University of Wisconsin-Madison

Early life social learning is enhanced by interactions with effectively designed environments. Four events were held at different city parks using inexpensive, temporary waterslides to stimulate social learning. This study describes the prototypes used and the evaluation of the children's interactions.

Bioengineering

Differentiation of Human Induced Pluripotent Stem Cells to Brain Microvascular Endothelial Cell-Like Cells with a Mature Immune Phenotype

Kinya Matsuo¹, Britta Engelhardt², Hideaki Nishihara³

¹Graduate School of Medicine, Yamaguchi University, ²Theodor Kocher Institute, University of Bern, ³Department of Neurotherapeutics, Yamaguchi University of Medicine

Here, we describe a protocol, the extended endothelial cell culture method (EECM), that allows differentiation of pluripotent stem cells to brain microvascular endothelial cell (BMEC)-like cells. These cells show endothelial cell adhesion molecule expression and are thus a human blood-brain barrier model suitable to study immune cell interactions in vitro.