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Lund University Hospital

2 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
Aaron C. Brown 1, Ulrika Blank 2, Derek C. Adams 1, Michele J. Karolak 1, Jennifer L. Fetting 1, Beth L. Hill 1, Leif Oxburgh 1
1Department of Molecular Medicine, Maine Medical Center Research Institute, 2Molecular Medicine and Gene Therapy, Lund University Hospital

In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.

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Neuroscience

Transplantation of Human Stem Cell-Derived GABAergic Neurons into the Early Postnatal Mouse Hippocampus to Mitigate Neurodevelopmental Disorders
Ana Gonzalez-Ramos 2, Kerstin Laurin 1, Fredrik Berglind 1, Marco Ledri 3, Merab Kokaia 2, My Andersson 1
1Cellular Neurophysiology and Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 2Experimental Epilepsy Group, Epilepsy Centre, Department of Clinical Sciences, Lund University Hospital, 3Molecular Neurophysiology and Epilepsy Group, Epilepsy Center, Department of Clinical Sciences, Lund University Hospital

Transplantation of human pluripotent stem cell-derived GABAergic neurons generated by neuronal programming could be a potential treatment approach for neurodevelopmental disorders. This protocol describes the generation and transplantation of human stem cell-derived GABAergic neuronal precursors into the brains of neonatal mice, allowing long-term investigation of grafted neurons and evaluation of their therapeutic potential.

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