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Mahidol University

7 ARTICLES PUBLISHED IN JoVE

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Medicine

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model
Panithi Sukho 1,2,3, Geesien S.A. Boersema 4, Nicole Kops 5, Johan F. Lange 4, Jolle Kirpensteijn 1,6, Jan Willem Hesselink 1, Yvonne M. Bastiaansen-Jenniskens 5, Femke Verseijden 1,5
1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 2Department of Otorhinolaryngology, Erasmus MC University Medical Center, 3Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, 4Department of Surgery, Erasmus MC University Medical Center, 5Department of Orthopaedics, Erasmus MC University Medical Center, 6Hill's Pet Nutrition Inc.

The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

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Medicine

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
Sirada Srihirun 1,2, Alan N. Schechter 2, Barbora Piknova 2
1Department of Pharmacology, Faculty of Dentistry, Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

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Biology

Isolation of Human Neutrophils from Whole Blood and Buffy Coats
Alan Y. Hsu 1, Zhicheng Peng 1, Hongbo Luo 1, Fabien Loison 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

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Biology

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay
Chalermpon Kumpitak 1, Wang Nguitragool 2, Liwang Cui 3, Jetsumon Sattabongkot 1, Sirasate Bantuchai 4
1Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, 2Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine, Mahidol University, 3Department of Internal Medicine, Morsani College of Medicine, University of South Florida, 4Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University

This protocol describes a sandwich enzyme-linked immunosorbent assay to detect salivary gland sporozoites in mosquitoes. Using easily available monoclonal antibodies, the method enables cost-effective, high-throughput detection of mosquitoes carrying Plasmodium falciparum or Plasmodium vivax. The method is suitable for malaria transmission research, including vector surveys.

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Biochemistry

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Khanit Sa-ngiamsuntorn 1, Piyanoot Thongsri 2, Yongyut Pewkliang 2, Suparerk Borwornpinyo 3,4, Adisak Wongkajornsilp 5
1Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, 4Department of Biotechnology, Faculty of Science, Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

We present a protocol to screen anti-hepatitis B virus (HBV) compounds targeting pre- and post-viral entry lifecycle stages, using isothermal titration calorimetry to measure binding affinity (KD) with host sodium taurocholate cotransporting polypeptide. Antiviral efficacy was determined through the suppression of viral lifecycle markers (cccDNA formation, transcription, and viral assembly).

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A Video Repository for Innovative Methods of Dietary Assessment and Analysis
Sabri Bromage 1,2, Teresa T. Fung 3, Sharon I. Kirkpatrick 4, Walter C. Willett 2
1Institute of Nutrition, Mahidol University, 2Department of Nutrition, Harvard T. H. Chan School of Public Health, 3Department of Nutrition, Simmons University, 4School of Public Health Sciences, University of Waterloo

A Video Repository for Innovative Methods of Dietary Assessment and Analysis

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Behavior

Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice
Supawadee Jariyasakulroj 1,2, Qing Chang 1, Pao-Fen Ko 1, Yang Shu 1, Ziying Lin 1, Jingyi Chen 1, Jian-Fu Chen 1
1Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, 2Department of Masticatory Science, Faculty of Dentistry, Mahidol University

This protocol describes how measuring bite force and head withdrawal threshold can capture pain behavior in Complete Freund's Adjuvant (CFA)-induced inflammatory temporomandibular joint (TMJ) pain.

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