University of Richmond

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti

¹Department of Biology, University of Richmond

A simplified yet accurate method to collect and stain mosquito hemocytes is described. Our method combines the simplicity of perfusion with the accuracy of high injection techniques to isolate clean preparations of hemocytes in Aedes mosquitoes. This method facilitates studies requiring knowledge of the types of hemocytes and their abundance.

Bioengineering

Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry

Tran T. Doan¹, Michael H. Freeman², Adrienne R. Schmidt², Natalie D. T. Nguyen², Michael C. Leopold¹

¹Department of Chemistry, Gottwald Center for the Sciences, University of Richmond, ²Department of Biochemistry and Molecular Biology, Gottwald Center for the Sciences, University of Richmond

Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).