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Paul Langerhans Institute Dresden

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Isolation of Human Islets from Partially Pancreatectomized Patients
Gregor Bötticher 1, Dorothèe Sturm 1, Florian Ehehalt 1, Klaus P. Knoch 2, Stephan Kersting 1, Robert Grützmann 1, Gustavo B. Baretton 3, Michele Solimena 2, Hans D. Saeger 1
1Department of GI-, Thorax- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology, Paul Langerhans Institute Dresden, 3Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden

The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

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Medicine

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
Dorothée Sturm 1,2, Lorella Marselli 3, Florian Ehehalt 1,2, Daniela Richter 1, Marius Distler 2, Stephan Kersting 1,2, Robert Grützmann 2, Krister Bokvist 4, Philippe Froguel 5, Robin Liechti 6, Anne Jörns 7, Paolo Meda 8, Gustavo Bruno Baretton 9, Hans-Detlev Saeger 2, Anke M. Schulte 10, Piero Marchetti 3, Michele Solimena 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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