University of Salamanca

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Extracellular Recording of Neuronal Activity Combined with Microiontophoretic Application of Neuroactive Substances in Awake Mice

Yaneri A. Ayala¹, David Pérez-González¹, Daniel Duque^1,2, Alan R. Palmer³, Manuel S. Malmierca^1,4

¹Auditory Neuroscience Laboratory, Institute of Neuroscience of Castilla y León, University of Salamanca, ²Neural Systems Laboratory, Institute for Systems Research, University of Maryland, ³Medical Research Council Institute of Hearing Research, ⁴Department of Cell Biology and Pathology, Faculty of Medicine, University of Salamanca

We present methods for the construction of electrodes to simultaneously record extracellular neural activity and release multiple neuroactive substances at the vicinity of the recording sites in awake mice. This technique allows the detailed analysis of putative local synaptic inputs to the neuron of interest.

Behavior

Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain

Verónica Lamas^1,2, Sheila Estévez¹, Marianni Pernía¹, Ignacio Plaza¹, Miguel A. Merchán¹

¹Institute of Neuroscience of Castilla y León, University of Salamanca, ²Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Department of Otolaryngology, Harvard Medical School

We describe a method for the stereotactically-guided location, exposure, and ablation of the auditory cortex in rats. The localization of the ablation is assessed using a coordinate map postmortem.