Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo FibroblastsMarc D. Bullock 1,2, Max Mellone 1, Karen M. Pickard 1, Abdulkadir Emre Sayan 1, Richard Mitter 3, John N. Primrose 2, Graham K. Packham 1, Gareth Thomas 1, Alexander H. Mirnezami 1,2
1Cancer Sciences Unit, University of Southampton School of Medicine, 2University Surgical Unit, University of Southampton School of Medicine, 3Bioinformatics Unit, London Research Institute, Cancer Research UK
Molecular profiling of laser microdissected cells and stroma from synthetic, tissue-engineered colorectal cancer models represents a novel and manipulatable approach to characterize and dissect the distinctive biology at the interface between tumor cells at the invasive front and cancer associated stromal cells.
