Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron MicroscopyRicha Aishwarya 1, Chowdhury S. Abdullah 1, Naznin Sultana Remex 2, Sadia Nitu 1, Brandon Hartman 1, Judy King 1, Md. Shenuarin Bhuiyan 1,2
1Department of Pathology and Translational Pathobiology, Louisiana State University Health Sciences Center-Shreveport, 2Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center-Shreveport
The present protocol describes a method of immuno-labeling a protein in the heart tissue sections using quantum dots. This technique provides a useful tool to visualize any protein's subcellular localization and expression at the ultrastructural level.
