Effective Lysis of Cyanobacterial and Green Algal Single Cells for Whole Genome Amplification in MicrofluidicsYuguang Liu 1, Dirk Schulze-Makuch 2, Jean-Pierre de Vera 3, Charles Cockell 4, Thomas Leya 5, Mickael Baque 3, Marina Walther-Antonio 1,6
1Department of Surgery, Division of Surgical Research, Mayo Clinic, 2Astrobiology Group, Center of Astronomy and Astrophysics, Technical University, 3Management and Infrastructure, Astrobiological Laboratories, German Aerospace Center (DLR), Institute of Planetary Research, 4School of Physics and Astronomy, University of Edinburgh, 5Branch Bioanalytics and Bioprocesses (IZI-BB), Extremophile Research & Biobank CCCryo, Fraunhofer Institute for Cell Therapy and Immunology, 6Department of Obstetrics and Gynecology, Mayo Clinic
Here we present a protocol to lyse cyanobacteria and green algae single cells that allows for subsequent single-cell whole genome amplification in a microfluidic platform with a 100% success rate.
