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University of delhi

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds
Marc E. Bracke 1, Bart I. Roman 2, Christian V. Stevens 2, Liselot M. Mus 1, Virinder S. Parmar 3, Olivier De Wever 1, Marc M. Mareel 1
1Department of Radiation Oncology and Experimental Cancer Research, University of Ghent, 2Department of Sustainable Organic Chemistry and Technology, University of Ghent, 3Department of Chemistry, University of Delhi

Here, we present a protocol to study the invasion of tumor cells into living normal tissue fragments in three dimensions. This organ culture technique is mainly applied to test potentially anti-invasive drugs in vitro.

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Biochemistry

Demonstration of Membrane Transport of Histidine using Goat Intestinal Inverted Sacs: An Experiential Pedagogical Tool for Undergraduates
Hiba Haris 1, Prachi Kumar 1, Vivek Bhardwaj 1, Sidhartha Taritla 1, Vandana Malhotra 1, Nandita Narayanasamy 1
1Department of Biochemistry, Sri Venkateswara College, University of Delhi

Here, we report an inexpensive and reproducible method demonstrating the membrane transport of histidine in a goat intestine. This process occurs by co-transporting histidine and sodium ions enabled by the sodium gradient across the enterocyte membrane. This method exploits experiential learning pedagogy to better understand solute movement across biological membranes.

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Chemistry

Green Synthesis of Quinoline-Based Ionic Liquid
Kajal Sharma 1, Meenakshi Sharma 1
1Molecular Genetics of Aging, Dr. B.R. Ambedkar Center for Biomedical Research (ACBR), University of Delhi

In the present work, we elucidate the green synthesis of quinoline-based Ionic Liquid (IL), namely, 1-Hexadecylquinolin-1-ium bromide {[C16quin]Br} by mixing quinoline with an excess of 1-Bromohexadecane, along with its detailed characterization using Nuclear Magnetic Resonance and Infrared spectroscopic measurements.

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