Lovelace Respiratory Research Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice

Maria E. Laucho-Contreras¹, Katherine L. Taylor¹, Ravi Mahadeva², Steve S. Boukedes³, Caroline A. Owen^1,4

¹Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital - Harvard Medical School, ²Department of Respiratory Medicine, University of Cambridge - Addenbrooke's Hospital, ³Lung Transplant Program, Brigham and Women's Hospital - Harvard Medical School, ⁴COPD and IPF Programs, Lovelace Respiratory Research Institute

The goal of this protocol is to provide automated methods to quantify chronic lung pathologies in a murine model of COPD. The protocol includes exposing mice to cigarette smoke (CS), measuring pulmonary function, inflating the lungs, and using morphometry methods to measure emphysema and small airway remodeling in mice.

Environment

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Neil D. Sanscrainte¹, Christy M. Waits^2,3, Christopher J. Geden¹, Alden S. Estep^1,2, James J. Becnel¹

¹USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, ²CMAVE Detachment, Navy Entomology Center of Excellence, ³Lovelace Respiratory Research Institute

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.