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Renmin hospital of Wuhan university

6 ARTICLES PUBLISHED IN JoVE

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Biology

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Monica F. Poelchau 1, Xin Huang 1, Allison Goff 1, Julie Reynolds 2, Peter Armbruster 1
1Department of Biology, Georgetown University, 2Insect Physiology Lab, EEOB, The Ohio State University

RNA-Seq analyses are becoming increasingly important for identifying the molecular underpinnings of adaptive traits in non-model organisms. Here, a protocol to identify differentially expressed genes between diapause and non-diapause Aedes albopictus mosquitoes is described, from mosquito rearing, to RNA sequencing and bioinformatics analyses of RNA-Seq data.

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Biology

High-throughput Measurement of Gut Transit Time Using Larval Zebrafish
Steven Cassar 1, Xin Huang 2, Todd Cole 3
1Preclinical Safety, AbbVie, 2Statistics, AbbVie, 3Integrated Science and Technology, AbbVie

The goal of this protocol is to measure the transit time of fluorescently labeled food through the gut of larval zebrafish in a high throughput fashion.

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Cancer Research

Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
Yuan Wen 1, Xie Song-ping 2, Liu Pan 3, Liu Xiao-yan 1, Pan Shan 4, Yin Qian 4, Sun Meng 5, Huang Xiao-xing 6, Xiao Rui-jing 4, Xiong Jie 4, Zhang Qiu-ping 4, Shao Liang 1
1Department of Hematology, Zhongnan Hospital of Wuhan University, 2Department of Thoracic Surgery, Renmin Hospital of Wuhan University, 3Department of Human Anatomy, Histology and Embryology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 4Department of Immunology, School of Basic Medical Sciences, Wuhan University, 5Department of Gastroenterology, Central Hospital of Wuhan, 6Department of Transfusion, Zhongnan Hospital of Wuhan University

ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.

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Neuroscience

Mapping Regional Homogeneity and Functional Connectivity of the Visual Cortex in Resting-State fMRI
Xin Huang *1, Yan Tong *1, Chen-Xing Qi 1, Yang-Tao Xu 1, Han-Dong Dan 1, Qin-Qin Deng 1, Yin Shen 1
1Eye Center, Renmin Hospital of Wuhan University

We present a protocol for analyzing functional magnetic resonance imaging data to investigate spontaneous neural activity alterations in retinitis pigmentosa patient using a combined regional homogeneity and functional connectivity method.

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Cancer Research

Three Differential Expression Analysis Methods for RNA Sequencing: limma, EdgeR, DESeq2
Shiyi Liu *1, Zitao Wang *1, Ronghui Zhu 1, Feiyan Wang 2, Yanxiang Cheng 1, Yeqiang Liu 2
1Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, 2Department of Pathology, Shanghai Skin Disease Hospital, Tongji University School of Medicine

A detailed protocol of differential expression analysis methods for RNA sequencing was provided: limma, EdgeR, DESeq2.

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Neuroscience

Methanol-Based Whole-Mount Preparation for the Investigation of Retinal Ganglion Cells
Ningzhi Zhang 1, Zhiyi Wang 1, Pei Lin 1, Yiqiao Xing 1, Ning Yang 1
1Department of Ophthalmology, Renmin Hospital of Wuhan University

Methanol can be used as an auxiliary fixed medium for retinal whole-mount preparations and long-term storage, which is useful for the investigation of retinal ganglion cells.

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