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The Royal Veterinary College

2 ARTICLES PUBLISHED IN JoVE

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Medicine

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy
Jayesh Dudhia 1, Patricia Becerra 2, Miguel A. Valdés 2, Francisco Neves 2, Neil G. Hartman 3, Roger K.W. Smith 1
1Department of Clinical Science and Services, The Royal Veterinary College, 2Hospital de Referencia La Equina, Manilva, 3Department of Nuclear Medicine, Barts & The London NHS Trust

This protocol describes the radiolabeling of equine mesenchymal stem cells and their implantation into tendon injuries in the horse in order to determine cell survival and tissue distribution using gamma scintigraphy.

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Immunology and Infection

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
Yogesh Singh 1,2, Oliver A. Garden 3, Florian Lang 2, Bradley S. Cobb 1
1Department of Comparative Biomedical Sciences, The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services, The Royal Veterinary College

Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.

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