The Royal Veterinary College

2 ARTICLES PUBLISHED IN JoVE

Medicine

In Vivo Imaging and Tracking of Technetium-99m Labeled Bone Marrow Mesenchymal Stem Cells in Equine Tendinopathy

Jayesh Dudhia¹, Patricia Becerra², Miguel A. Valdés², Francisco Neves², Neil G. Hartman³, Roger K.W. Smith¹

¹Department of Clinical Science and Services, The Royal Veterinary College, ²Hospital de Referencia La Equina, Manilva, ³Department of Nuclear Medicine, Barts & The London NHS Trust

This protocol describes the radiolabeling of equine mesenchymal stem cells and their implantation into tendon injuries in the horse in order to determine cell survival and tissue distribution using gamma scintigraphy.

Immunology and Infection

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Yogesh Singh^1,2, Oliver A. Garden³, Florian Lang², Bradley S. Cobb¹

¹Department of Comparative Biomedical Sciences, The Royal Veterinary College, ²Institute of Physiology I, Cardiology & Vascular Medicine, Eberhard Karls University of Tuebingen, ³Department of Clinical Science and Services, The Royal Veterinary College

Many experimental systems have been utilized to understand the mechanisms regulating T cell development and function in an immune response. Here a genetic approach using retroviral transduction is described, which is economic, time efficient, and most importantly, highly informative in identifying regulatory pathways.