University of Texas Medical School

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Cut-loading: A Useful Tool for Examining the Extent of Gap Junction Tracer Coupling Between Retinal Neurons

Hee Joo Choi¹, Christophe P. Ribelayga², Stuart C. Mangel¹

¹Department of Neuroscience, Ohio State University College of Medicine, ²Department of Ophthalmology and Visual Science, University of Texas Medical School

An easy and convenient method to determine the extent of gap junction tracer coupling between retinal neurons is described. This technique enables one to investigate the function of the electrical synapses between neurons in the intact retina under different illumination conditions and at different times of the day and night.

Biology

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

Jennifer L. Abrams¹, Kevin A. Morano¹

¹Department of Microbiology and Molecular Genetics, Graduate School of Biomedical Sciences, University of Texas Medical School

This article describes the use of a firefly luciferase-GFP fusion protein to investigate in vivo protein folding in Saccharomyces cerevisiae. Using this reagent, refolding of a model heat-denatured protein can be monitored simultaneously by fluorescence microscopy and an enzymatic assay to probe the roles of proteostasis network components in protein quality control.