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Muhlenberg College

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
Keri L Colabroy 1, Katlyn Mayer 1
1Department of Chemistry, Muhlenberg College

Here we present a protocol for the benchtop immobilized metal affinity chromatography purification and subsequent reconstitution of a polyhistidine tagged, non-heme iron binding dioxygenase suitable for the undergraduate teaching laboratory.

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