Manipulating Mechanical Forces in the Developing Zebrafish Heart Using Magnetic BeadsChristina Vagena-Pantoula 1,2, Hajime Fukui 3,4, Julien Vermot 1,2
1Department of Bioengineering, Imperial College London, 2The Francis Crick Institute, 3Institute of Advanced Medical Sciences, Tokushima University, 4Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute
This protocol outlines a method for grafting a magnetic bead into the developing zebrafish heart through microsurgery, enabling the manipulation of mechanical forces in vivo and triggering mechanical stimulus-dependent calcium influx in endocardial cells.
