Chungbuk National University

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fluorescence detection methods for microfluidic droplet platforms

Xavier Casadevall i Solvas¹, Xize Niu¹, Katherine Leeper¹, Soongwon Cho¹, Soo-Ik Chang², Joshua B. Edel¹, Andrew J. deMello³

¹Department of Chemistry, Imperial College London , ²Department of Biochemistry, Protein Chip Research Center, Chungbuk National University, ³Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich

Droplet-based microfluidic platforms are promising candidates for high throughput experimentation since they are able to generate picoliter, self-compartmentalized vessels inexpensively at kHz rates. Through integration with fast, sensitive and high resolution fluorescence spectroscopic methods, the large amounts of information generated within these systems can be efficiently extracted, harnessed and utilized.

Immunology and Infection

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Jun-O Jin^1,2, Soyeong Jang³, Hyehyun Kim⁴, Junghwan Oh⁴, Sungbo Shim⁵, Minseok Kwak^3,4, Peter C.W. Lee⁶

¹Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, ²Department of Medical Biotechnology, Yeungnam University, ³Department of Chemistry, Pukyong National University, ⁴Marine-integrated Bionics Research Center, Pukyong National University, ⁵Department of Biochemistry, Chungbuk National University, ⁶Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

Experimental procedures for the subsequent extraction of lymphatic tissues to test lymphoid dendritic cell activation are described after treatment of an immunostimulating nanomaterial.

Bioengineering

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Se Hee Lee^*1, Ho Won Lee^*2, Da Som Kim², Hyuck Gi Kwon³, Jong Hyun Lee³, Yang-Hoon Kim¹, Ok Chan Jeong^2,3, Ji-Young Ahn¹

¹College of Natural Sciences, Chungbuk National University, ²Department of Biomedical Engineering, Inje University, ³Institute of Digital Anti-Aging Healthcare, Inje University

This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.

Chemistry

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks (MOFs)

Jeehwan Han^*1, Seongwoo Kim^*2, Mi Sun Lee¹, Min Kim², Nakcheol Jeong¹

¹Department of Chemistry, Korea University, ²Department of Chemistry and BK21Plus Research Team, Chungbuk National University

Here, we present a protocol for active site validation of metal-organic framework catalysts by comparing stoichiometric and catalytic carbonyl-ene reactions to find out whether a reaction takes place on the inner or outer surface of metal-organic frameworks.

Chemistry

Synthesis of Triazole and Tetrazole-Functionalized Zr-Based Metal-Organic Frameworks Through Post-Synthetic Ligand Exchange

Sangho Lee^*1, Daeyeon Lee^*1, Jin Yeong Kim², Min Kim¹

¹Department of Chemistry, Chungbuk National University, ²Department of Chemistry Education, Seoul National University

Post-synthetic ligand exchange (PSE) is a versatile and powerful tool for installing functional groups into metal-organic frameworks (MOFs). Exposing MOFs to solutions containing triazole- and tetrazole-functionalized ligands can incorporate these heterocyclic moieties into Zr-MOFs through PSE processes.