JoVE Logo

Sign In

Loma Linda University

12 ARTICLES PUBLISHED IN JoVE

image

Medicine

Biochemical Measurement of Neonatal Hypoxia
Megan S. Plank 1, Teleka C. Calderon 1, Yayesh Asmerom 2, Danilo S. Boskovic 1, Danilyn M. Angeles 2
1Division of Biochemistry, Department of Basic Sciences, Loma Linda University, 2Division of Physiology, Department of Basic Sciences, Loma Linda University

A method is described to measure biochemical markers of neonatal hypoxia-ischemia. The approach utilizes high pressure liquid chromatography (HPLC) and Gas Chromatography Mass Spectrometry (GC/MS).

image

Medicine

The Use of Thermal Infra-Red Imaging to Detect Delayed Onset Muscle Soreness
Hani H. Al-Nakhli 1, Jerrold S. Petrofsky 1,2, Michael S. Laymon 2, Lee S. Berk 1
1Loma Linda University, 2Azusa Pacific University

The purpose of this investigation was to assess whether using an infra-red thermal camera is a valid tool for detecting and quantifying the muscle soreness after exercising.

image

Biology

A Rapid In Vivo Bioassay for Developmentally Active Enhancers
Charmaine U. Pira 1, Shelley A. Caltharp 2, Endika Haro 1, Kerby C. Oberg 1
1Department of Pathology and Human Anatomy, Loma Linda University, 2Department of Pathology, Children's Healthcare of Atlanta

With the increased use of transcription factor-specific ChIP-seq technology, techniques to validate potential regulatory regions are critical. The authors describe a rapid chick bioassay to validate the activity of regulatory sequences functioning during development.

image

Developmental Biology

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Wei Wen 1, Jian-Ping Zhang 1, Wanqiu Chen 2, Cameron Arakaki 2, Xiaolan Li 1,2, David Baylink 2, Gary D. Botimer 3, Jing Xu 1, Weiping Yuan 1, Tao Cheng 1,4,5,6,7, Xiao-Bing Zhang 1,2
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 3Department of Orthopaedic Surgery, Loma Linda University, 4Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, 5Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, 6Collaborative Innovation Center for Cancer Medicine, 7Tianjin Key Laboratory of Blood Cell Therapy and Technology

This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.

image

Medicine

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
Jacqueline S. Coats 1, Ineavely Baez 1, Cornelia Stoian 1, Terry-Ann M. Milford 2, Xiaobing Zhang 3, Olivia L. Francis 1, Ruijun Su 1, Kimberly J. Payne 1
1Department of Pathology and Human Anatomy, Loma Linda University, 2Department of Basic Sciences, Loma Linda University, 3Department of Medicine, Loma Linda University

Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.

image

Immunology and Infection

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
Yi Xu *1, Samiksha Wasnik *1, David J. Baylink 1, Edmundo Carreon Berumen 1, Xiaolei Tang 1
1Department of Medicine, Division of Regenerative Medicine, Loma Linda University

Qa-1 (HLA-E in human) belongs to a group of non-classical major histocompatibility complex 1b molecules. Immunization with Qa-1-binding epitopes has been shown to augment tissue-specific immune regulation and ameliorate several autoimmune diseases. Herein we describe an overlapping peptide library strategy for the identification of Qa-1 epitopes in a protein.

image

The Efficacy of a Porcine Collagen Matrix in Keratinized Tissue Augmentation: A 6-Month Follow-up Study
Carlo Maiorana 1, Luca Pivetti 1, Fabrizio Signorino 1, Giovanni Battista Grossi 1, Mario Beretta 1, Alan Scott Herford 2
1Implant Center for Edentulism and Jawbone Atrophies, Maxillofacial Surgery and Odontostomatology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, 2Oral and Maxillofacial Surgery Department, Loma Linda University

The aim of this study was to evaluate the long-term stability of the results achieved in peri implant tissue grafting by means of a collagen matrix.

image

Biology

Simultaneous Measurements of Intracellular Calcium and Membrane Potential in Freshly Isolated and Intact Mouse Cerebral Endothelium
Md A. Hakim 1, Erik J. Behringer 1
1Department of Basic Sciences, Loma Linda University

Demonstrated here are protocols for (1) freshly isolating intact cerebral endothelial "tubes" and (2) simultaneous measurements of endothelial calcium and membrane potential during endothelium-derived hyperpolarization. Further, these methods allow for pharmacological tuning of endothelial cell calcium and electrical signaling as individual or interactive experimental variables.

image

Neuroscience

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
Samantha B. Palahnuk 1,2, Jonathan A. Abdala 1, Vadim V. Gospodarev 3, Christopher G. Wilson 1
1Center for Perinatal Biology, Department of Basic Sciences, Loma Linda University, 2Department of Biology, The College of New Jersey, 3Department of Neurosurgery, Loma Linda University

This protocol both visually communicates the brainstem-spinal cord preparation and clarifies the preparation of brainstem transverse slices in a comprehensive step-by-step manner. It was designed to increase reproducibility and enhance the likelihood of obtaining viable, long lasting, rhythmically-active slices for recording neural output from the respiratory regions of the brainstem.

image

Immunology and Infection

In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Hongzheng Bi 1,2, Samiksha Wasnik 1, David J. Baylink 1, Chenfan Liu 1,3,4, Xiaolei Tang 1,3
1Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 2Zhengzhou University, 3Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, Long Island University, 4Jinan Infectious Disease Hospital, Shandong University

Here we present a protocol for in vivo augmentation of gut-homing regulatory T cell induction. In this protocol, dendritic cells are engineered to locally produce high concentrations of the active vitamin D (1,25-dihydroxyvitamin D or 1,25[OH]2D) and the active vitamin A (retinoic acid or RA) de novo.

image

Biology

Assessment of Global Ocular Structure Following Spaceflight Using a Micro-Computed Tomography (Micro-CT) Imaging Method
Gina D. Roque-Torres 1, Nina C. Nishiyama 2, Seta Stanbouly 2, Xiao Wen Mao 2
1Center for Dental Research, Loma Linda University, 2Department of Basic Sciences, Division of Biomedical Engineering Sciences (BMES), Loma Linda University

We present a protocol using high-resolution micro-computed tomography imaging to determine whether spaceflight induced damage on ocular structures. The protocol shows the micro-CT-derived measurement of ex vivo rodent ocular structures. We demonstrate the ability to assess ocular morphological changes following spaceflight using a non-destructive tridimensional technique to evaluate ocular damage.

image

Biology

Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma
Md A. Hakim 1, Paulo W. Pires 2, Erik J. Behringer 1
1Department of Basic Sciences, Loma Linda University, 2Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center, University of Arizona College of Medicine Tucson

Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved