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National Physical Laboratory

6 ARTICLES PUBLISHED IN JoVE

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Biology

Test Samples for Optimizing STORM Super-Resolution Microscopy
Daniel J. Metcalf 1, Rebecca Edwards 1, Neelam Kumarswami 1, Alex E. Knight 1
1Analytical Science Division, National Physical Laboratory

We describe the preparation of three test samples and how they can be used to optimize and assess the performance of STORM microscopes. Using these examples we show how to acquire raw data and then process it to acquire super-resolution images in cells of approximately 30-50 nm resolution.

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Tip-Enhanced Raman Spectroscopy (TERS) Imaging of Grapheme
Andrew Pollard 1, Naresh Kumar 1, Weitao Su 2, Debdulal Roy 1
1National Physical Laboratory, 2Institute of Material Physics, Hangzhou Dianzi University

A comprehensive methodology is described for overcoming experimental obstacles encountered when using tip-enhanced Raman spectroscopy (TERS) to map the properties of graphene surfaces. Critical steps, such as the optical alignment of the system, the preparation of TERS probes and the operational TERS procedure required to obtain consistent results, are outlined.

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Biology

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
Louise E. Bird 1, Heather Rada 1, Anil Verma 1, Raphael Gasper 2,3, James Birch 4, Matthew Jennions 4, Jan Lӧwe 3, Isabel Moraes 4, Raymond J. Owens 1
1Oxford Protein Production Facility, Research Complex at Harwell, 2Protein Crystallography Group, Ruhr University, 3MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, 4Membrane Protein Laboratory, Diamond Light Source

A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is presented.

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Environment

Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization
Inder Kaur 1, Laura-Jayne Ellis 1, Isabella Romer 1, Ratna Tantra 2, Marie Carriere 3,4, Soline Allard 5, Martine Mayne-L'Hermite 5, Caterina Minelli 6, Wolfgang Unger 7, Annegret Potthoff 8, Steffi Rades 7, Eugenia Valsami-Jones 1
1School of Geography, Earth and Environmental Sciences, University of Birmingham, 2Analytical Science, National Physical Laboratory, 3INAC-LCIB, Université Grenoble Alpes, 4CEA, INAC-SyMMES, 5NIMBE, CEA, CNRS, Université Paris-Saclay, CEA Saclay, 6Chemical, Medical and Environmental Science, National Physical Laboratory, 7BAM Division 6.1 'Surface Analysis and Interfacial Chemistry', BAM Federal Institute for Materials Research and Testing, 8Fraunhofer Institute for Ceramic Technologies and Systems

Here, we present a step-wise protocol for the dispersion of nanomaterials in aqueous media with real-time characterization to identify the optimal sonication conditions, intensity, and duration for improved stability and uniformity of nanoparticle dispersions without impacting the sample integrity.

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Biochemistry

High-Throughput Protein Crystallization via Microdialysis
Tristan O. C. Kwan 1, Amy E. Danson 1, Pascelle Draper 2, Paul Reardon 2, Isabel Moraes 1
1National Physical Laboratory, 2SWISSCI LTD

The presented protocol describes a straightforward approach for screening protein crystallization conditions and crystal growth using a 96-well high-throughput dialysis plate. The use of dialyzer tubes for the large-scale growth of microcrystals is also demonstrated for serial crystallography and MicroED applications.

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Medicine

A Stable Phantom Material for Optical and Acoustic Imaging
Lina Hacker 1,2, Aoife M. Ivory 3, James Joseph 4,5, Janek Gröhl 1,2, Bajram Zeqiri 3, Srinath Rajagopal 3, Sarah E. Bohndiek 1,2
1Department of Physics, University of Cambridge, 2Cancer Research UK Cambridge Institute, University of Cambridge, 3Ultrasound and Underwater Acoustics Group, Department of Medical, Marine and Nuclear Physics, National Physical Laboratory, 4School of Science and Engineering, University of Dundee, 5Centre for Medical Engineering and Technology, University of Dundee

This protocol describes the fabrication of a stable, biologically relevant phantom material for optical and acoustic biomedical imaging applications, featuring independently tunable acoustic and optical properties.

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