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Henan Agricultural University

1 ARTICLES PUBLISHED IN JoVE

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Biology

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells
Jingna Yang 1, Fangyuan Kang 1, Anqi Wei 1, Wenyan Lu 1, Xinfu Zhang 1, Liqiang Han 1
1College of Veterinary Medicine, Henan Agricultural University

The present protocol describes how to use oil red O to dye lipid droplets (LDs), calculate the size and number of LDs in a fatty acid-induced fatty hepatocyte model, and use BODIPY 493/503 to observe the process of small LDs fusing into large LDs by live cell imaging.

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