INSTITUTO POLITÉCNICO NACIONAL

4 ARTICLES PUBLISHED IN JoVE

Environment

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Claudia Guerrero-Barajas¹, Alberto Ordaz¹, Selene Montserrat García-Solares¹, Claudio Garibay-Orijel¹, Fernando Bastida-González², Paola Berenice Zárate-Segura¹

¹Bioprocesses Department, Laboratory of Environmental Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, ²Laboratory of Molecular Biology, Escuela Superior de Medicina, Instituto Politécnico Nacional

Microbial sulfate reduction is a process of great importance in environmental biotechnology. The success of the sulfidogenic reactors depends among other factors on the microbial composition of the sludge. Here, we present a protocol to develop sulfidogenic sludge from hydrothermal vents sediments in a UASB reactor for reductive dechlorination purposes.

Chemistry

Determining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation

Monika S. Walczak¹, Perla Morales-Gil^1,2, Turia Belashehr¹, Kiran Kousar¹, Paulina Arellanes Lozada^1,3, Robert Lindsay¹

¹Corrosion and Protection Centre, School of Materials, The University of Manchester, ²Laboratorio de Caracterización de Materiales Sintéticos y Naturales, Instituto Mexicano del Petróleo, ³Departamento de Metalurgia y Materiales, Instituto Politécnico Nacional

A protocol to avoid the oxidation of metallic substrates during sample transfer from an inhibited acidic solution to an X-ray photoelectron spectrometer is presented.

Biochemistry

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Lucero Romero-Aguilar^*1,2, Mónica Montero-Lomeli^*3, Juan Pablo Pardo², Guadalupe Guerra- Sánchez¹

¹Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ²Facultad de Medicina, Universidad Nacional Autónoma de México, ³Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro

Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis.

Medicine

Evaluation of Hydration Status by Bioelectrical Impedance Vector Analysis in Patients with Ischemic Heart Disease Undergoing Exercise Stress Test

Pineda-Juárez Juan Antonio¹, Navarrete-de la O Dulce María¹, Suárez-Cuenca Juan Antonio², Lara-Vargas Jorge Antonio³, Arteaga-Martínez José Rodolfo³, Radillo-Alba Hugo Alberto³, Leyva-Valadez Eduardo Alfonso³, Morales-Portano Julieta Danira⁴, Gómez-Álvarez Enrique⁴, Santellano-Juárez Brenda⁵, Mondragón-Terán Paul⁶

¹Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research, Centro Médico Nacional “20 de Noviembre”, ISSSTE, ²Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Centro Médico Nacional “20 de Noviembre”, ISSSTE, ³Cardiac Rehabilitation Service, Centro Médico Nacional “20 de Noviembre”, ISSSTE, ⁴Cardiology Service, Centro Médico Nacional “20 de Noviembre“, ISSSTE, ⁵Georgia Cancer Center, ⁶Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, CICATA-Unidad Morelos, Instituto Politécnico Nacional

Hydration status imbalances can have a short-term effect on direct and indirect determinants of oxygen uptake and pulse, and morbidity and mortality prognostic factors in ischemic heart disease. This protocol describes the technique for assessment of hydration status through bioelectrical impedance vector analysis and cardiopulmonary response during exercise stress test.