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Medizinische Hochschule Hannover

3 ARTICLES PUBLISHED IN JoVE

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Biology

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
Verónica A. Lombardo 1, Anje Sporbert 1, Salim Abdelilah-Seyfried 1
1Max Delbrück Center for Molecular Medicine

Here, we present a method for the photoactivated switch of photoconvertible fluorescent proteins (PCFPs) in the living zebrafish embryo and further tracking of photoconverted protein at specific time points during development. This methodology allows monitoring of cell biological events underlying different developmental processes in a live vertebrate organism.

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Biology

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
Cécile Otten 1, Salim Abdelilah-Seyfried 1
1Max Delbrück Center for Molecular Medicine

The method presented here comprises the precise injury of live zebrafish embryos with high-energy laser pulses and the subsequent analysis of these injuries and their recovery with time. We also show how genetically labeled single or groups of skeletal muscle cells can be tracked during and after laser light induced damage.

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Developmental Biology

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
Verónica A. Lombardo *1,2,3, Cécile Otten *1,2, Salim Abdelilah-Seyfried 1,2,3
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology, University of Potsdam, 3Institute of Molecular Biology, Medizinische Hochschule Hannover

To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

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