Norwich Research Park

2 ARTICLES PUBLISHED IN JoVE

Biology

Identification of Post-translational Modifications of Plant Protein Complexes

Sophie J. M. Piquerez¹, Alexi L. Balmuth², Jan Sklenář², Alexandra M.E. Jones^1,2, John P. Rathjen³, Vardis Ntoukakis¹

¹School of Life Sciences, University of Warwick, ²The Sainsbury Laboratory, Norwich Research Park, ³Research School of Biology, The Australian National University

We describe here a protocol for the purification and characterization of plant protein complexes. We demonstrate that by immunoprecipitating a single protein within a complex, so we can identify its post-translational modifications and its interacting partners.

Genetics

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Joanna M. Feehan^*1,2, Katherine E. Scheibel^*1, Salim Bourras³, William Underwood⁴, Beat Keller³, Shauna C. Somerville¹

¹Department of Plant and Microbial Biology, University of California Berkeley, ²John Innes Centre, Norwich Research Park, ³Department of Plant and Microbial Biology, University of Zürich, ⁴USDA-ARS Sunflower and Plant Biology Research Unit

Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.