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University of Rome Tor Vergata

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Mechanical and Controlled PRP Injections in Patients Affected by Androgenetic Alopecia
Pietro Gentile 1,2, Simone Garcovich 3, Maria Giovanna Scioli 4, Alessandra Bielli 4, Augusto Orlandi 5, Valerio Cervelli 6
1Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata, 2Chief of Teaching of Plastic and Reconstructive Surgery, Catholic University, 3Dermatology Department, Catholic University, 4Anathomic Pathology Department, University of Rome Tor Vergata, 5Chief of Anathomic Pathology Department, University of Rome Tor Vergata, 6Chief of Plastic and Reconstructive Surgery Department, University of Rome Tor Vergata

This paper aims to describe the interfollicular autologous platelet-rich plasma injections (0.20 mL x cm2) in the scalp of 23 patients affected by androgenetic alopecia at a depth of 5 mm using a medical injector gun equipped with a 30 gauge needle, in three sessions.

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Immunology and Infection

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells
Alessandra Ventura *1,2, Aaron Vassall *1, Alp Yurter 1, Eve Robinson 1, Renata Filler 1, Douglas Hanlon 1, Katrina Meeth 1, Harib Ezaldein 1, Michael Girardi 1, Olga Sobolev 1, Marcus W. Bosenberg 1,3, Richard L. Edelson 1
1Department of Dermatology, Yale University School of Medicine, 2Dermatology Department, University of Rome Tor Vergata, 3Department of Pathology, Yale University School of Medicine

The transimmunization (TI) device or plate and related protocols have been developed to replicate the key features of extracorporeal photochemotherapy (ECP), in an experimental setting, allowing for production of physiologically activated, tunable dendritic cells (DCs) for cancer immunotherapy.

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Chemistry

Phase Change Dimethyldioctadecylammonium-Shelled Microdroplets as a Promising Drug Delivery System: Results on 3D Spheroids of Mammalian Tumor Cells
Elisabetta Tortorella *1,2, Damiano Palmieri *1, Martina Piermarini 1, Daniele Gigante 1, Letizia Oddo 1, Yosra Toumia 1, Gaio Paradossi 1, Fabio Domenici 1
1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, 2Department of Molecular Medicine, Sapienza University of Rome

Decafluoropentane microdroplets developed with a shell of dimethyldioctadecylammonium bromide exhibited an exceptional colloidal stability and an actractive biointerface. DDAB-MDs proved to be efficient drug reservoirs characterized by a high affinity to plasma membranes together with enhanced uptake and antitumor activity of Doxorubicin against human triple-negative breast cancer (MDA-MB-231) 3D model.

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