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RIKEN Research Center for Allergy and Immunology

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Application of a Mouse Ligated Peyer’s Patch Intestinal Loop Assay to Evaluate Bacterial Uptake by M cells
Shinji Fukuda 1, Koji Hase 1, Hiroshi Ohno 1
1Laboratory for Epithelial Immunobiology, RIKEN Research Center for Allergy and Immunology

M cells in a specialized follicle-associated epithelium covering Peyer’s patches play an important role for the mucosal immunosurveillance in gut-associated lymphoid tissue. Here we described the evaluation method for bacterial transcytosis by M cells in vivo. This method provides a method to understand M-cell function in the immune system.

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