Max Planck Institute for Neurological Research

3 ARTICLES PUBLISHED IN JoVE

Medicine

Mangala Srinivas¹, Philipp Boehm-Sturm², Markus Aswendt², Eberhard D. Pracht^2,3, Carl G. Figdor¹, I. Jolanda de Vries¹, Mathias Hoehn²

¹Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, ²In-Vivo-NMR Laboratory, Max Planck Institute for Neurological Research, ³German Center for Neurodegenerative Diseases (DZNE)

We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.

Neuroscience

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)

Shuheng Liu^*1, Katharina Tielking^*1, Dario von Wedel¹, Melina Nieminen-Kelhä¹, Susanne Mueller^2,3, Philipp Boehm-Sturm^2,3, Peter Vajkoczy¹, Ran Xu¹

¹Department of Neurosurgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health, ²Department of Experimental Neurology and Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, ³NeuroCure Cluster of Excellence and Charité Core Facility 7T Experimental MRIs, Charité - Universitätsmedizin Berlin

Here we present a standardized SAH mouse model, induced by endovascular filament perforation, combined with magnetic resonance imaging (MRI) 24 h after operation to ensure the correct bleeding site and exclude other relevant intracranial pathologies.

Biology

Terminal H-reflex Measurements in Mice

Frederique Wieters^*1, Matthias Gruhn^*2, Ansgar Büschges², Gereon R. Fink^1,3, Markus Aswendt¹

¹Faculty of Medicine and University Hospital Cologne, Department of Neurology, University of Cologne, ²Department for Animal Physiology, Institute for Zoology, Biocenter Cologne, University of Cologne, ³Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Center Juelich

The clinical evaluation of spasticity based on the Hoffmann reflex (H-reflex) and using electrical stimulation of peripheral nerves is an established method. Here, we provide a protocol for a terminal and direct nerve stimulation for H-reflex quantification in the mouse forepaw.