Establishment of an Extracellular Acidic pH Culture SystemAyano Kondo 1, Tsuyoshi Osawa 2
1Innovative Technology Laboratories, Kyowa Hakko Kirin Co., Ltd, 2Laboratory for Systems Biology and Medicine, RCAST, The University of Tokyo
The acidic tumor microenvironment plays critical roles in tumor progression. To assess the effects of acidic extracellular pH on cancer cells in vitro, we established simple acidic culture systems.