Rice University

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analysis of Neural Crest Migration and Differentiation by Cross-species Transplantation

¹Department of Biochemistry and Cell Biology, Rice University

An approach for analyzing migration and eventual fate of avian neural crest cells in quail-chick chimeric embryos is described. This method is a simple and straightforward technique for tracing neural crest cells during migration and differentiation that are otherwise difficult to distinguish within an unmanipulated chick embryo.

Immunology and Infection

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Alexandra Bili Yin¹, David Hawke², Dapeng Zhou^3,4

¹Undergraduate Program, Rice University , ²Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center , ³Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center , ⁴University of Texas Graduate School of Biological Sciences at Houston

A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.

Engineering

Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor

Victoria Astley¹, Kimberly Reichel¹, Rajind Mendis¹, Daniel M. Mittleman¹

¹Department of Electrical and Computer Engineering, Rice University

The procedure for implementing a refractive index sensor for terahertz frequencies based on a grooved parallel-plate waveguide geometry is described here. The method yields a measurement of the refractive index of a small volume of liquid through monitoring of the shift in the resonant frequency of the waveguide structure

JoVE Journal

Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy

Stuart J. Corr^1,2, Brandon T. Cisneros^1,2, Leila Green¹, Mustafa Raoof¹, Steven A. Curley^1,3

¹Department of Surgical Oncology, University of Texas M.D. Anderson Cancer Center, ²Department of Chemistry, Rice University , ³Mechanical Engineering and Materials Science, Rice University

We describe the protocols used to investigate the interactions of 13.56 MHz radiofrequency (RF) electric-fields with gold nanoparticle colloids in both non-biological and biological systems (in vitro/vivo). These interactions are being investigated for applications in cancer therapy.