University of Wisconsin - Milwaukee

4 ARTICLES PUBLISHED IN JoVE

Biology

Cell Block Preparation from Cytology Specimen with Predominance of Individually Scattered Cells

¹Department of Pathology, University of Wisconsin - Milwaukee

Shidham's method for preparation of cell blocks with AV-marker from cytology specimens containing individually scattered cells and small cell groups.

Biology

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Vinod B. Shidham¹, George M. Varsegi¹, Krista D'Amore¹, Anjani Shidham²

¹Department of Pathology, University of Wisconsin - Milwaukee, ²BioInnovation LLC

Practicing of fine needle aspiration biopsies (FNAB) by trainees is relatively challenging, due to the lack of an easily available, appropriate lesion. Preparation of an AV phantom lesion for practicing the FNAB procedure and mastering proficiency is relatively easy.

Medicine

Performing and Processing FNA of Anterior Fat Pad for Amyloid

Vinod B. Shidham^1,2, Bryan Hunt¹, Safwan S. Jaradeh³, Alexandru C. Barboi³, Sumana Devata⁴, Parameswaran Hari⁵

¹Department of Pathology, Medical College of Wisconsin, ²Current Address: Department of Pathology, Wayne State University School of Medicine Detroit Medical Center, ³Department of Neurology, Medical College of Wisconsin, ⁴Department of Medicine, Medical College of Wisconsin, ⁵Division of Neoplastic Diseases and Related Disorders, Medical College of Wisconsin

Fat pad aspiration is a preferred, minimally invasive, and low cost approach as compared to other methods to detect amyloid for diagnosis of systemic amyloidosis. This video article demonstrates a procedural outline for performing fat pad aspiration with appropriate processing of the specimen for the optimal diagnostic outcome.

Biology

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Michael Stoneman¹, Deo Singh¹, Valerica Raicu^1,2

¹Department of Physics, University of Wisconsin - Milwaukee, ²Department of Biological Sciences, University of Wisconsin - Milwaukee

By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.