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Heilongjiang University of Chinese Medicine

2 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Anthony A. High 1, Haiyan Tan 1, Vishwajeeth R. Pagala 1, Mingming Niu 2,3, Ji-Hoon Cho 1, Xusheng Wang 1, Bing Bai 2, Junmin Peng 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

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Behavior

Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction
Fang Wan *1, Zhifang Guo *2,3, Mingye Wang 4, Yunlong Hou 5,6, Lu Wang 3,5, Wenyan Li 4,7, Ning Kang 2,3, Pengyu Zhu 8, Mengnan Li 5,6
1Heilongjiang University of Chinese Medicine, 2Hebei Provincial Key Laboratory of Luobing, 3Key Laboratory of State Administration of TCM (Cardio-Cerebral Vessel Collateral Disease), 4Hebei University of Chinese Medicine, 5Shijiazhuang Compound Traditional Chinese Medicine Technology Innovation Center, 6National Key Laboratory of Luobing Research and Innovative Chinese Medicine, 7Shijiazhuang Yiling Pharmaceutical Co., Ltd, 8The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Here, we describe a protocol for implementing mild anesthesia and acupuncture treatment on a chronic hypoxia mouse model and conducting behavioral tests to assess the cognitive alterations post-treatment.

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