Heilongjiang University of Chinese Medicine

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Anthony A. High¹, Haiyan Tan¹, Vishwajeeth R. Pagala¹, Mingming Niu^2,3, Ji-Hoon Cho¹, Xusheng Wang¹, Bing Bai², Junmin Peng^1,2

¹St. Jude Proteomics Facility, St. Jude Children's Research Hospital, ²Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, ³Heilongjiang University of Chinese Medicine

We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

Behavior

Acupuncture Treatment in a Mouse Model of Chronic Hypoxia-Induced Cognitive Dysfunction

Fang Wan^*1, Zhifang Guo^*2,3, Mingye Wang⁴, Yunlong Hou^5,6, Lu Wang^3,5, Wenyan Li^4,7, Ning Kang^2,3, Pengyu Zhu⁸, Mengnan Li^5,6

¹Heilongjiang University of Chinese Medicine, ²Hebei Provincial Key Laboratory of Luobing, ³Key Laboratory of State Administration of TCM (Cardio-Cerebral Vessel Collateral Disease), ⁴Hebei University of Chinese Medicine, ⁵Shijiazhuang Compound Traditional Chinese Medicine Technology Innovation Center, ⁶National Key Laboratory of Luobing Research and Innovative Chinese Medicine, ⁷Shijiazhuang Yiling Pharmaceutical Co., Ltd, ⁸The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Here, we describe a protocol for implementing mild anesthesia and acupuncture treatment on a chronic hypoxia mouse model and conducting behavioral tests to assess the cognitive alterations post-treatment.