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University of Otago

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
Urszula Grabiec *1, Tim Hohmann *1, Niels Hammer 2, Faramarz Dehghani 1
1Department of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 2Department of Anatomy, University of Otago

Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.

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Neuroscience

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
Faraz Ahmad 1, Ping Liu 1
1Department of Anatomy, School of Biomedical Sciences, University of Otago

We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.

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Biology

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
Golnoush Madani *1, Erwin Lamping *1, Hee Ji Lee 1, Masakazu Niimi 1,2, Alok K. Mitra 3, Richard D. Cannon 1
1Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, 2Department of Microbiology, Faculty of Medicine, Chulalongkorn University, 3School of Biological Sciences, University of Auckland

This article presents a small-scale plasma membrane isolation protocol for the characterization of Candida albicans ABC (ATP-binding cassette) protein Cdr1, overexpressed in Saccharomyces cerevisiae. A protease-cleavable C-terminal mGFPHis double tag with a 16-residue linker between Cdr1 and the tag was designed to facilitate the purification and detergent-screening of Cdr1.

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Immunology and Infection

Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
Matloob Husain 1
1Department of Microbiology and Immunology, University of Otago

Influenza A virus (IAV) infection activates the caspases that cleave host and viral proteins, which, in turn, have pro- and antiviral functions. By employing inhibitors, RNA interference, site-directed mutagenesis, and western blotting and RT-qPCR techniques, caspases in infected mammalian cells that cleave host cortactin and histone deacetylases were identified.

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