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University of New Mexico Health Sciences Center

4 ARTICLES PUBLISHED IN JoVE

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Medicine

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development
Russell A. Morton 1, Marvin R. Diaz 1, Lauren A. Topper 1, C. Fernando Valenzuela 1
1Department of Neurosciences, School of Medicine, University of New Mexico Health Sciences Center

We demonstrate the construction of alcohol vapor chambers using readily available materials that simultaneously house 6 mouse cages. We further describe their use in a mouse model of fetal alcohol exposure equivalent to all 3 trimesters of human pregnancy. This paradigm exposes animals during gestation and postnatal days 1-12.

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Biochemistry

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Elizabeth M. Bailey 1, Emanuel Salazar-Cavazos 1, Rachel M. Grattan 1, Michael J. Wester 2, David J. Schodt 2, Julian A. Rojo 1, Keith A. Lidke 2,3, Diane S. Lidke 1,3
1Department of Pathology, University of New Mexico School of Medicine, 2Department of Physics and Astronomy, University of New Mexico, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center

The present protocol describes sample preparation and data analysis to quantify protein phosphorylation using an improved single-molecule pull-down (SiMPull) assay.

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Behavior

Urokinase-type Plasminogen Activator-induced Mouse Back Pain Model
Marena A. Montera 1, Aleyah E. Goins 1, Sascha R. A. Alles 1, Karin N. Westlund 1
1Department of Anesthesiology & Critical Care Medicine, University of New Mexico Health Sciences Center

Methods for simple, rapid induction of a back pain model in mice are provided here using an intraligament injection of urinary plasminogen activator.

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Immunology and Infection

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
Ziming Cao 1, Matthew J. Garcia 2, Larry A. Sklar 2,3,4,5, Angela Wandinger-Ness 3,4, Zhichao Fan 1
1Department of Immunology, School of Medicine, UConn Health, 2Center for Molecular Discovery, University of New Mexico Health Sciences Center, 3Comprehensive Cancer Center, University of New Mexico Health Sciences Center, 4Department of Pathology, University of New Mexico Health Sciences Center, 5Autophagy, Inflammation, & Metabolism (AIM) Center, University of New Mexico

This protocol describes a flow cytometry-based, high-throughput screening method to identify small-molecule drugs that inhibit β2 integrin activation on human neutrophils.

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