Medical Research Council
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
Fabrice Gorrec 1, Jan Löwe 1
1Laboratory of Molecular Biology, Medical Research Council
Automated systems and protocols for the routine preparation of a large number of screens and nanoliter crystallization droplets for vapor diffusion experiments are described and discussed.
Biochemistry
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
Filip Pamula 1,2, Jonas Mühle 1, Alain Blanc 3, Rony Nehmé 4, Patricia C. Edwards 4, Christopher G. Tate 4, Ching-Ju Tsai 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council
This report describes screening of different detergents for preparing the visual GPCR, rhodopsin, and its complex with mini-Go. Biochemical methods characterizing the quality of the complex at different stages during purification are demonstrated. This protocol can be generalized to other membrane protein complexes for their future structural studies.
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