University of Toledo, College of Pharmacy and Pharmaceutical Sciences

2 ARTICLES PUBLISHED IN JoVE

Medicine

Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease

Wissam A. AbouAlaiwi¹, Ingrid Rodriguez², Surya M. Nauli¹

¹Department of Pharmacology, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, ²Department of Emergency and Intensive Care, ProMedica Sponsored Research

Spectral Karyotyping (SKY) is an advanced cytogenetics technique to identify genomic and chromosomal aberrations. This technique takes advantage of chromosome painting probes, which allow classification of all chromosomes. SKY can also identify complex chromosome aberrations and segregation defects in mice and humans with various diseases, including polycystic kidney disease.

Neuroscience

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Alzahra J. Al Omran¹, Hannah C. Saternos¹, Tongyu Liu², Surya M. Nauli³, Wissam A. AbouAlaiwi¹

¹Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, ²Life Sciences Institute, University of Michigan, ³Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus

Using high-resolution differential interference contrast (DIC) microscopy, an ex vivo observation of the beating of motile ependymal cilia located within the mouse brain ventricles is demonstrated by live-imaging. The technique allows a recording of the unique ciliary beating frequency and beating angle as well as their intracellular calcium oscillation pacing properties.