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Ludwig Maximilian University of Munich

5 ARTICLES PUBLISHED IN JoVE

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JoVE Core

20 mJ, 1 ps Yb:YAG Thin-disk Regenerative Amplifier
Ayman Alismail 1,2, Haochuan Wang 1,3, Jonathan Brons 3, Hanieh Fattahi 1,3
1Department of Physics, Ludwig Maximilian University of Munich, 2Physics and Astronomy Department, King Saud University, 3Max Planck Institute of Quantum Optics

A protocol for the operation of a high-energy, high-power optical parametric chirped pulse amplifier pump source based on an Yb:YAG thin-disk regenerative amplifier is presented here.

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Genetics

A Customizable Protocol for String Assembly gRNA Cloning (STAgR)
Christopher T. Breunig 1,2, Andrea M. Neuner 1,2, Jessica Giehrl-Schwab 3, Wolfgang Wurst 3, Magdalena Götz 2,4, Stefan H. Stricker 1,2
1MCN Junior Research Group, Munich Center for Neurosciences, Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum, German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum, German Research Center for Environmental Health, 4Physiological Genomics, Ludwig Maximilian Universitat, BioMedical Center

Here, we present string assembly gRNA cloning (STAgR), a method to easily multiplex gRNA vectors for CRISPR/Cas9 approaches. STAgR makes gRNA multiplexing simple, efficient and customizable.

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Medicine

Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique
Martin Ryll 1, Julian Bucher 1, Moritz Drefs 1, Florian Bösch 1, K. Kumaraswami 2, Tobias Schiergens 1, Hanno Niess 1, Markus Schoenberg 1, Sven Jacob 1, Markus Rentsch 1, Markus Guba 1, Jens Werner 1, Joachim Andrassy 1, Michael N. Thomas 1,3
1Department of General, Visceral, and Transplant Surgery, Ludwig Maximilian University of Munich, 2Walter-Brendel-Centre of Experimental Medicine, Ludwig Maximilian University of Munich, 3Department of General, Visceral and Cancer Surgery, University Hospital of Cologne, University of Cologne

Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its formation.

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Neuroscience

Cryo-section Dissection of the Adult Subependymal Zone for Accurate and Deep Quantitative Proteome Analysis
Christian Friess 1, Magdalena Götz 1,2,3, Jacob Kjell 1,2,4
1Division of Physiological Genomics, Biomedical Center, Ludwig Maximilian University of Munich, 2Institute for Stem Cell Research, Helmholtz Zentrum München, 3SYNERGY, Excellence Cluster Systems Neurology, University of Munich, 4Department of Clinical Neuroscience, Karolinska Institutet

Cryo-section-dissection allows fresh, frozen preparation of the largest neurogenic niche in the murine brain for deep quantitative proteome analysis. The method is precise, efficient, and causes minimal tissue perturbation. Therefore, it is ideally suited for studying the molecular microenvironment of this niche, as well as other organs, regions, and species.

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Biology

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
Jessica Radmer 1, Johanna Geuder 1, Fiona C. Edenhofer 1, Wolfgang Enard 1, Mari Ohnuki 1,2,3
1Faculty of Biology, Ludwig Maximilian University of Munich, 2Institute for the Advanced Study of Human Biology, Kyoto University, 3Hakubi Center, Kyoto University

The present protocol describes a method to isolate, expand, and reprogram human and non-human primate urine-derived cells to induced pluripotent stem cells (iPSCs), as well as instructions for feeder-free maintenance of the newly generated iPSCs.

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