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Kasetsart University

5 ARTICLES PUBLISHED IN JoVE

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Environment

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
Pamela Nicholson 1, Pattarasuda Rawiwan 2, Win Surachetpong 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

This protocol diagnoses Tilapia Lake Virus (TiLV) in tilapia tissues using RT-PCR methodologies. The entire method is described from tissue dissection to total RNA extraction, followed by cDNA synthesis and detection of TiLV by either conventional PCR or quantitative PCR using dsDNA binding a fluorescent binding dye.

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Immunology and Infection

Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Assay for the Specific and Rapid Detection of Tilapia Lake Virus
Theerawut Phusantisampan 1, Pattarasuda Rawiwan 2,3, Sri Rajiv Kumar Roy 2, Malinee Sriariyanun 4, Win Surachetpong 2,3
1Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North Bangkok (KMUTNB), 2Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, 3Center for Advanced Studies for Agriculture and Food, Institute for Advanced Studies, Kasetsart University, 4Department of Chemical and Process Engineering, The Sirindhorn Thai-German International Graduate School of Engineering (TGGS), King Mongkut's University of Technology North Bangkok (KMUTNB)

We present an RT-LAMP assay for the detection of TiLV in tilapia fish using simple instruments over a relatively short period of time compared to conventional RT-PCR techniques. This protocol may help control the epidemic spread of TiLVD, especially in developing countries.

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Bioengineering

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Sougata Ghosh 1,2,3, Tanay Bhagwat 3, Rohini Kitture 4, Sirikanjana Thongmee 1, Thomas J. Webster 3,5,6,7
1Department of Physics, Faculty of Science, Kasetsart University, 2Department of Microbiology, School of Science, RK. University, 3Department of Chemical Engineering, Northeastern University, 4Defence Institute of Advanced Technology Campus, Navyukti Innovations Pvt. Ltd., 5Department of Biomedical Engineering, Hebei University of Technology, 6Department of Chemistry, Saveetha School of Engineering, Institute of Medical and Technical Science, Saveetha University, 7UFPI - Universidade Federal do Piauí

Novel nanocomposites of graphene nanoribbons and hydroxyapatite nanoparticles were prepared using solution-phase synthesis. These hybrids when employed in bioactive scaffolds can exhibit potential applications in tissue engineering and bone regeneration.

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Biology

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs
Mohammad Asmat-Ullah 1, Rusydi Rozaimi 1, Hanafiah Fazhan 1,2,3, Alexander Chong Shu-Chien 2,4, Youji Wang 5, Khor Waiho 1,2,3
1Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 2Centre for Chemical Biology, Universiti Sains Malaysia, 3Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 4School of Biological Sciences, Universiti Sains Malaysia, 5International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University

Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate.

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Biology

Surgical Technique of the 3-Dimensional-printed Personalized Hip Implant for the Treatment of Canine Hip Dysplasia
Irin Kwananocha 1,2, Femke Verseijden 1, Seyed A Kamali 1, Joëll Magré 3, Koen Willemsen 3, Jacobine CM Schouten 4, Daniela Salvatori 4, Marianna A Tryfonidou 1, Björn P Meij 1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, 2Research and Academic Service, Faculty of Veterinary Medicine, Kasetsart University, 3Department of Orthopedics, University Medical Center Utrecht, 4Department of Clinical Sciences, Anatomy and Physiology, Faculty of Veterinary Medicine, Utrecht University

This work describes a novel surgical technique for extracapsular implantation of a personalized, 3-dimensional-printed, joint-preserving implant. This novel treatment aims to restore hip stability in young adult dogs suffering from hip dysplasia with laxity by uniquely reproducing the anatomical shape of the acetabular rim of the hip joint.

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