Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System Madhumita Sridharan 1, Tripthi Battapadi 1, Lata Balakrishnan 1
1Department of Biology, Indiana University Indianapolis
This article provides a procedure for the affinity purification of a human recombinant protein, flap endonuclease 1 (FEN1), which has been labeled with a 6X-histidine tag. The protocol involves the utilization of two distinct immobilized metal ion columns for the purification of the tagged protein.
