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San Raffaele Scientific Institute

6 ARTICLES PUBLISHED IN JoVE

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Medicine

A Simplified Technique for In situ Excision of Cornea and Evisceration of Retinal Tissue from Human Ocular Globe
Mohit Parekh 1, Stefano Ferrari 1, Enzo Di Iorio 1, Vanessa Barbaro 1, Davide Camposampiero 1, Marianthi Karali 2, Diego Ponzin 1, Gianni Salvalaio 1
1Fondazione Banca Degli Occhi del Veneto O.N.L.U.S. , 2Telethon Institute for Genetics & Medicine (T.I.G.E.M.)

The paper describes a simplified technique to excise corneal and to eviscerate retinal tissues from the ocular globe of human cadaveric donors. The technique described here will help to excise good quality tissues to be used for transplantation, surgical or research purposes without damaging other tissues of the ocular globe.

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Immunology and Infection

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
Marcella Facchini 1,2, Ida De Fino 1,2, Camilla Riva 1,2, Alessandra Bragonzi 1
1Infections and Cystic Fibrosis Unit, Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute, 2Italian Cystic Fibrosis Research Foundation

We describe the agar-beads method to establish persistent long-term chronic Pseudomonas aeruginosa airway infection in the mouse model.

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Developmental Biology

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
Ombretta Guardiola 1, Gennaro Andolfi 1, Mario Tirone 2,3, Francescopaolo Iavarone 1, Silvia Brunelli 2, Gabriella Minchiotti 1
1Stem Cell Fate Laboratory, Institute of Genetics and Biophysics "A. Buzzati Traverso", CNR, 2School of Medicine and Surgery, University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases, San Raffaele Scientific Institute

This manuscript describes a detailed protocol to induce acute skeletal muscle regeneration in adult mice and subsequent manipulations of the muscles, such as dissection, freezing, cutting, routine staining, and myofiber cross-sectional area analysis.

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JoVE Journal

Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion
Giorgia Careccia *1,2, Federica Colombo *1,3, Mario Tirone 1, Alessandra Agresti 1, Marco E. Bianchi 1,2, Samuel Zambrano *1,2, Emilie Vénéreau *1
1Chromatin Dynamics Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3Department of Electronics, Information and Bioengineering, Politecnico di Milano

Skeletal muscle differentiation is a highly dynamic process, which particularly relies on nuclear positioning. Here, we describe a method to track nuclei movements by live cell imaging during myoblast differentiation and myotube formation and to perform a quantitative characterization of nuclei dynamics by extracting information from automatic tracking.

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Medicine

Fertility Sparing Procedure using Carbon Dioxide Fiber Laser Vaporization of Ovarian Endometrioma
Massimo Candiani 1, Jessica Ottolina 1, Iacopo Tandoi 1, Ludovica Bartiromo 1, Matteo Schimberni 1, Roberta Villanacci 1, Stefano Ferrari 1
1Gynecological Surgery and Endometriosis Departmental Unit, San Raffaele Scientific Institute

In this protocol, CO2 fiber laser technique is demonstrated for the surgical treatment of ovarian endometriosis, which represents a viable alternative in terms of fertility preservation with the major advantage of not being dependent on the surgeon's skills and personal experience.

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Medicine

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome
Valeria Stella Vanni *1,2, Alessandra Alteri *1,2, Lucia De Santis 1,2, Greta Chiara Cermisoni 1, Elisa Rabellotti 1, Diana Delprato 1, Marta Parma 1, Enrico Papaleo 1, Luigi Fedele 3, Massimo Candiani 1,2
1Obstetrics and Gynaecology Unit, IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University, 3Department of Obstetrics and Gynecology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico

A dedicated team can offer Mayer-Rokitansky-Kuster-Hauser patients the option to perform controlled ovarian stimulation and oocyte cryopreservation at the time of laparoscopic vaginoplasty.

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