Universiti Malaya

7 ARTICLES PUBLISHED IN JoVE

Cancer Research

Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Noorzaileen Eileena Zaidi¹, Nur Aima Hafiza Shazali², Adam Leow Thean Chor¹, Mohd Azuraidi Osman¹, Kamariah Ibrahim³, Marilyn Jaoi-Edward⁴, Nik Mohd Afizan Nik Abd Rahman^1,2

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, ²Institute of Tropical Forestry and Forestry Products (INTROP), Universiti Putra Malaysia, ³Department of Biomedical Science, Faculty of Medicine, University of Malaya, ⁴Agro-Biotechnology Institute (ABI), National Institute of Biotechnology Malaysia (NIBM)

Macrophage phagocytic activity against cancer cells, specifically 4T1 mouse mammary carcinoma cells, was imaged in this study. The live cell coculture model was established and observed using a combination of fluorescent and differential interference contrast microscopy. This assessment was imaged using imaging software to develop multipoint time-lapse video.

Medicine

Endoscopic Ultrasound-Guided Biliary Drainage: Endoscopic Ultrasound-Guided Hepaticogastrostomy in Malignant Biliary Obstruction

Stanley Khoo¹, Ida Hilmi¹, Jun Kit Koong², Peng Soon Koh², Boon Koon Yoong², Sanjiv Mahadeva¹

¹Gastroenterology and Hepatology Unit, Department of Medicine, Faulty of Medicine, University of Malaya, ²Hepato-Pancreato-Biliary Unit, Department of Surgery, Faculty of Medicine, University of Malaya

Endoscopic Ultrasound-Guided Biliary Drainage (EUS-BD) is an alternative method of biliary decompression in malignant biliary obstruction. Here we describe the technique of EUS guided-Hepaticogastrostomy (EUS-HGS) in a case of unresectable malignant hilar biliary obstruction.

Engineering

Quantifying Arms and Legs Contributions during Repetitive Electrically-Assisted Sit-To-Stand Exercise in Paraplegics: A Pilot Study

Musfirah Abd Aziz^1,2, Nur Azah Hamzaid^1,2, Nazirah Hasnan³

¹Biomechatronics and Neuroprosthetics Laboratory, Department of Biomedical Engineering, Faculty of Engineering, Universiti Malaya, ²Center for Applied Biomechanics (CAB), Department of Biomedical Engineering, Faculty of Engineering, Universiti Malaya, ³Department of Rehabilitation Medicine, Faculty of Medicine, Universiti Malaya

Arms contribution in Sit-To-Stand (SitTS) is determined by the legs' muscle condition. Several compensating strategies were discovered in efforts to achieve complete SitTS cycles. These findings triangulate the spinal cord injury (SCI) persons' biomechanical measures with their subjective feeling of load borne by both their limbs throughout the SitTS approaches.

Neuroscience

Locomotor Assessment of 6-Hydroxydopamine-induced Adult Zebrafish-based Parkinson's Disease Model

Naemah Md Hamzah¹, Siong Meng Lim¹, Yuganthini Vijayanathan^1,2, Fei Tieng Lim¹, Abu Bakar Abdul Majeed¹, Maw Pin Tan², Kalavathy Ramasamy¹

¹Collaborative Drug Discovery Research (CDDR) Group and Brain Degeneration and Therapeutics Group, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Selangor, Kampus Puncak Alam, ²Department of Medicine, Faculty of Medicine, Universiti Malaya

The present protocol describes the intracerebroventricular (ICV) injection of adult zebrafish with neurotoxic 6-hydroxydopamine (6-OHDA) at the ventral diencephalon (Dn) and the assessment of the impairment and subsequent recovery of swimming behavior postlesion by using the open tank test, which is accompanied by analysis using a video tracking software.

Biology

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Mathanakumara Ealam Selvan^*1, Kai Shen Lim^*1, Chee How Teo², Yat-Yuen Lim¹

¹Institute of Biological Sciences, Faculty of Science, Universiti Malaya, ²Centre for Research in Biotechnology for Agriculture (CEBAR), Universiti Malaya

The protocol submitted here explains the complete in silico pipeline needed to predict and functionally characterize circRNAs from RNA-sequencing transcriptome data studying host-pathogen interactions.

Immunology and Infection

Virus Propagation and Cell-Based Colorimetric Quantification

Jia-Yi Tan¹, Jo-Ern Wong¹, Nurhafiza Zainal², Sazaly AbuBakar¹, Kim-Kee Tan¹

¹Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Center of Excellence (HICoE), Universiti Malaya, ²Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya

The present protocol describes the propagation of Zika virus (ZIKV) in Vero African green monkey kidney cells and the quantification of ZIKV using cell-based colorimetric immunodetection methods in 24-well and 96-well (high throughput) formats.

Biology

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Jo-Ern Wong¹, Nurhafiza Zainal², Sazaly AbuBakar¹, Kim-Kee Tan¹

The current study describes a protocol to monitor the changes in human umbilical vein endothelial cells (HUVECs) during viral infection using a real-time cell analysis (RTCA) system.