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Uniformed Services University of the Health Sciences

7 ARTICLES PUBLISHED IN JoVE

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Biology

Imaging of Estrogen Receptor-α in Rat Pial Arterioles using a Digital Immunofluorescent Microscope
Niloofar Rezvani 1, Andrei V. Blokhin 1, Emil Zeynalov 1, Marguerite T. Littleton-Kearney 1
1Graduate School of Nursing, Uniformed Services University of the Health Sciences

The goal of this article is to demonstrate a method to optimize immunofluorescent detection of estrogen receptor-α (ERα) in rat pial arterial slices using a digital immunofluorescent microscope.

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Immunology and Infection

Measuring Local Anaphylaxis in Mice
Holly Evans 1, Kristin E. Killoran 1, Edward Mitre 1
1Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences

Allergic responses, characterized by the activation of mast cells and basophils, are driven by the cross-linking of IgE and release of proinflammatory mediators. A quantitative assessment of allergic responses can be achieved by using Evans Blue dye to monitor changes in vascular permeability after allergen challenge.

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Medicine

Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema
Emil Zeynalov 1, Susan M. Jones 1, J. Paul Elliott 2
1Neurotrauma Research, Swedish Medical Center, 2Neurosurgery, Colorado Brain and Spine Institute

Our studies have revealed that the beneficial effects of conivaptan are dependent on the method of delivery after experimental stroke in mice. We have developed a research protocol for delivery of the receptor blocker via IV catheter on stroke-evoked brain edema formation in mice.

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Developmental Biology

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Darya Karabasheva 1, Jeremy T. Smyth 1
1Department of Anatomy, Physiology, and Genetics, F. Edward Hébert School of Medicine, Uniformed Services University of the Health Sciences

The goal of this protocol is to analyze cell division in intact tissue by live and fixed cell microscopy using Drosophila meiotic spermatocytes. The protocol demonstrates how to isolate whole, intact testes from Drosophila larvae and early pupae, and how to process and mount them for microscopy.

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Neuroscience

Cerebrovascular Reactivity Measurement with Functional Near Infrared Spectroscopy
Franck Amyot 1, Cora Davis 1, Mike Sangobowale 4, Carol Moore 2, Erika Silverman 4, Amir Gandjbakhche 3, Ramon Diaz-Arrastia 4, Kimbra Kenney 1,2
1National Intrepid Center of Excellence, Walter Reed National Military Medical Center, 2Uniformed Services University of the Health Sciences, 3The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 4University of Pennsylvania Perelman School of Medicine

Presented here is a protocol for imaging and measurement of cerebrovascular reactivity in humans with functional Near Infrared Spectroscopy (fNIRS). fNIRS is a novel imaging modality that captures the concentration changes of hemoglobin species in the brain’s outermost cortex under specific stimuli.

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Medicine

Development of a Selective Aortic Arch Perfusion System in a Porcine Model of Exsanguination Cardiac Arrest
Marta J. Madurska 1, Hossam Abdou 1, Michael J. Richmond 1, Noha N. Elansary 1, Peng F. Wong 2, Todd E. Rasmussen 3, Thomas M. Scalea 1, Jonathan J. Morrison 1
1R Adams Cowley Shock Trauma Center, 2Department of Vascular Surgery, The James Cook University Hospital, 3Uniformed Services University of the Health Sciences

The goal of this protocol is to demonstrate a porcine exsanguination cardiac arrest model and a specifically built selective aortic arch perfusion circuit for translational research.

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Medicine

Measuring Cardiac Output in a Swine Model
Neerav Patel 1, Hossam Abdou 1, Joseph Edwards 1, Noha N. Elansary 1, Kelly Poe 1, Michael J. Richmond 1, Marta J. Madurska 1, Todd E. Rasmussen 2, Jonathan J. Morrison 1
1R Adams Cowley Shock Trauma Center, University of Maryland Medical System, 2Uniformed Services University of the Health Sciences

Thermodilution, pressure-volume loop catheters, and contrast ventriculography are reliable and accurate methods for determining cardiac physiology such as stroke volume and cardiac output in a laboratory setting in swine.

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