Slippery Rock University

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Hydrolysis of a Ni-Schiff-Base Complex Using Conditions Suitable for Retention of Acid-labile Protecting Groups

Cory A. Bontrager¹, Tanner J. Geibel¹, George A. Lengyel¹

¹Department of Chemistry, Slippery Rock University

Here, we present an efficient hydrolysis and subsequent Fmoc protection of an amino acid isolated from a Ni-Schiff-base complex. Hydrolysis conditions presented here are suitable for use when retention of acid-labile side-chain protecting groups is required. This technique may be adaptable to a variety of unnatural amino acid substrates.