Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay PlatformJason S. Lehmann 1, Amy Zhao 1, Binggang Sun 1, Weiping Jiang 1, Shaoquan Ji 1
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This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.
