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sogang university

3 ARTICLES PUBLISHED IN JoVE

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Biology

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
Seonghyun Lee 1, Kyubong Jo 1
1Department of Chemistry and Interdisciplinary Program of Integrated Biotechnology, Sogang University

We present an approach for visualizing fluorescent protein DNA binding peptide (FP-DBP)-stained large DNA molecules tethered on the polyethylene glycol (PEG) and avidin-coated glass surface and stretched with microfluidic shear flows.

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Biochemistry

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Sanggil Kim 1, Wooseok Ko 1, Hyunji Park 1, Hyun Soo Lee 1
1Department of Chemistry, Sogang University

Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).

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Biochemistry

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine (DOPA) and Its Application to Protein Conjugation
Sanggil Kim 1, Hyun Soo Lee 1
1Department of Chemistry, Sogang University

Here, we present a protocol for the genetic incorporation of L-dihydroxyphenylalanine biosynthesized from simple starting materials and its application to protein conjugation.

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