Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral ProteaseNatalie D. Stull 1, Andrew Breite 2, Robert McCarthy 2, Sarah A. Tersey 1, Raghavendra G. Mirmira 1,3,4
1Department of Pediatrics and the Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 2VITACYTE, LLC, 3Department of Medicine, Indiana University School of Medicine, 4Department of Cellular and Integrative Physiology, Indiana University School of Medicine
A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.
