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Katholieke Universiteit Leuven

3 ARTICLES PUBLISHED IN JoVE

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Medicine

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction
Ryan Hodges 1,2, Masayuki Endo 1, Andre La Gerche 3, Elisenda Eixarch 4,5, Philip DeKoninck 1, Vessilina Ferferieva 3, Jan D'hooge 3, Euan M. Wallace 2, Jan Deprest 1
1Division Woman and Child, Department Women, University Hospitals Leuven, 2The Ritchie Centre, Monash Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Victoria, Australia, 3Department of Cardiovascular Sciences, Katholieke Universiteit Leuven, 4Fetal and Perinatal Medicine Research Group, Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 5Maternal-Fetal Medicine Department, ICGON, Hospital Clínic, Universitat de Barcelona, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER)

We describe examination of fetal cardiac function with contemporary functional fetal echocardiography and fetoplacental Doppler ultrasound using the VisualSonics VEVO 2100 microultrasound in a surgically induced model of intrauterine fetal growth restriction in a rabbit.

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Medicine

Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus
Laura Ordovás *1, Ruben Boon *1, Mariaelena Pistoni 1, Yemiao Chen 1, Rangarajan Sambathkumar 1, Nicky Helsen 1, Jolien Vanhove 1, Pieter Berckmans 1, Qing Cai 1, Kim Vanuytsel 1, Susanna Raitano 1, Catherine M. Verfaillie 1
1Stem Cell Institute, Katholieke Universiteit Leuven

Here we report a rapid and efficient gene editing method based on RMCE in the AAVS1 locus of human Pluripotent Stem Cells (hPSCs) that improves upon previously described systems. Using this technique, isogenic lines can be rapidly and reliably generated for proper comparative studies, facilitating transgenesis-mediated research with hPSCs.

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Neuroscience

Investigating Object Representations in the Macaque Dorsal Visual Stream Using Single-unit Recordings
Irene Caprara 1, Peter Janssen 1,2, Maria C. Romero 1
1Laboratorium voor Neuro-en Psychofysiologie, Katholieke Universiteit Leuven, 2The Leuven Brain Institute

A detailed protocol to analyze object selectivity of parieto-frontal neurons involved in visuomotor transformations is presented.

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