Ningxia medical university

5 ARTICLES PUBLISHED IN JoVE

Medicine

Observation of the Systolic Function of Isolated Right Atria from Guinea Pigs

Jiayuan Gu^*1, Zhengjun Zhang^*2, Jonnea Japhet Tibenda^*1, Lin Yan³, Yafei Zhu⁴, Qipeng Zhao^1,5,6

¹School of Pharmacy, Ningxia Medical University, ²Department of Cardiology, General Hospital of Ningxia Medical University, ³School of Basic Medical Sciences, Ningxia Medical University, ⁴College of Nursing, Ningxia Medical University, ⁵Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education Ningxia Medical University, ⁶Ningxia Regional Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Regional High Incidence Disease

The present protocol describes an efficient method for screening drugs that enhance myocardial contractility using isolated right atria from guinea pigs.

Neuroscience

Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection

Fuhan Fan¹, Hong Jiang¹, Ya Hou¹, Yi Zhang^1,2,3, Qipeng Zhao⁴, Yong Zeng^1,2, Xianli Meng^1,2,5, Xiaobo Wang²

¹School of Pharmacy/School of Modern Chinese Medicine Industry, Chengdu University of Traditional Chinese Medicine, ²Meishan Hospital of Chengdu University of Traditional Chinese Medicine, ³School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, ⁴School of Pharmacy, Ningxia Medical University, ⁵Innovative Institute of Chinese Medicine and Pharmacy/Academy for Interdiscipline, Chengdu University of Traditional Chinese Medicine

This protocol describes a dependable and efficient in vitro model of the brain blood barrier. The method uses mouse cerebral vascular endothelial cells bEnd.3 and measures transmembrane electrical resistance.

Medicine

Bioinformatics-based Analyses and B/Th Cell Epitope Prediction of Mugwort Pollen Allergen Art v 1 Protein

Jin Hu^*1,2,3, Xiangyu Zhou^*4, Yikun Cao^*4, Xiuli Cheng^*5, Na Li⁴, Bing Hua⁴, Hongwan Dang^1,3

¹School of Basic Medicine, Ningxia Medical University, ²Preparation Center, General Hospital of Ningxia Medical University, ³Department of Pharmacy, General Hospital of Ningxia Medical University, ⁴Institute of Neurological Diseases, Shizuishan First People's Hospital, ⁵Department of Pharmacy, People's Hospital of Ningxia Hui Autonomous Region

Here, we present a protocol to perform bioinformatics correlation analysis to predict the physicochemical properties, secondary structure, and B and T helper cell epitopes of mugwort pollen main allergen Art v 1 protein to provide a theoretical basis for the subsequent development of artemisia pollen allergic disease vaccine and disease treatment.

Neuroscience

Role of CD4 T Cell in Relapsing-Remitting Experimental Autoimmune Encephalomyelitis

Jin Hu^*1,2, Xiangyu Zhou^*3, Yikun Cao^*3, Huili Tian^*4, Na Li³, Xiuli Cheng⁵, Xiaoying Yang⁶, Hongwan Dang^1,6

¹School of Basic Medicine, Ningxia Medical University, ²Preparation Center, General Hospital of Ningxia Medical University, ³Institute of Neurological Diseases, First People's Hospital of Shizuishan, ⁴Department of Neurology, Xianyang Hospital of Yan’an University, ⁵Department of Pharmacy, People’s Hospital of Ningxia Hui Autonomous Region, ⁶Department of Pharmacy, General Hospital of Ningxia Medical University

An experimental autoimmune encephalomyelitis (EAE) model in mice was employed to investigate the role of CD4 T cells in the initial and relapse of EAE from the perspective of the activation phase and immune effect function.

Medicine

Sampling of N-methyl-N-nitrosourea-induced Rat's Retina Damage and Multi-index Evaluation of Pathological Changes

Yafei Zhu^*1, Xinlin Zhao^*1, Tibenda Jonnea Japhet¹, Jun Chen², Qipeng Zhao¹

¹School of Nursing, School of Pharmacy, Key Laboratory of Hui Ethnic Medicine Modernization, Ministry of Education, Ningxia Medical University, ²Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine

This protocol describes a procedure for rat retinal sampling and utilizing hematoxylin-eosin staining, TUNEL assay, and Western blot to detect pathological changes and apoptosis in the retina after intraperitoneal injection of N-Methyl-N-Nitrosourea.