An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence ImagesCarolina L. Haass-Koffler 1,2, Mohammad Naeemuddin 1, Selena E. Bartlett 1,3
1Medications Development, Ernest Gallo Clinic and Research Center, University of California, San Francisco , 2Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco , 3Translational Research Institute and the Institute for Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia
We developed a software platform that utilizes Imaris Neuroscience, ImarisXT and MATLAB to measure the changes in morphology of an undefined shape taken from three-dimensional confocal fluorescence of single cells. This novel approach can be used to quantify changes in cell shape following receptor activation and therefore represents a possible additional tool for drug discovery.
